Hepg2 2 oil red staining plamitate treatment
WebA. Oil red O staining of HepG2 cells. HepG2 cells were treated with rifampicin 5 µM (A-3), 10 µM (A-4), 20 µM (A-5) or TO901317 (10 µM, A-2) for 48 h. Cells treated with DMSO … Web11 apr. 2024 · what: In this study, on the basis of previous results, the authors show that GluOC decreases SCD1 by activating AMPK to alleviate hepatocyte lipid accumulation, and the authors also demonstrate that GPRC6A is the receptor for GluOC in HepG2 cells This study shows that the knockdown of SCD1 in OA/PA-induced hepatocytes reduces lipid …
Hepg2 2 oil red staining plamitate treatment
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Web13 apr. 2024 · 2.10 Oil red O staining. HCC cells were seeded into 24-well plates precoated with sterile cover slips and cultured overnight. Then, the cells were washed with fresh PBS and fixed with 4% formaldehyde. Subsequently, the cells were stained with oil red O solution (Sangon Biotech, Shanghai, China) for 1 h at room temperature. Web15 mei 2011 · HepG2 cells were co-incubated with various concentrations of FFAs for 24h and the intracellular lipid contents were observed by Oil Red O and Nile Red staining …
Web11 apr. 2024 · Oil Red O staining. The cells were plated and cultured to 70% density on coverslips placed in 24-well plates. The cells were fixed with 4% paraformaldehyde for 30 min, washed twice for 5 min with PBS, and stained with Oil Red O (Solarbio) working solution (stock solution/ddH2O, 3:2) for 10 min. Nuclei were stained using Harris. WebF2: Oil Red O staining for HepG2 cells and quantitative analysis of lipid. FFAs 1 mM for 24 h induced lipid accumulation in HepG2 cells. Visual observation of lipid content was …
WebTurmeric extract may inhibit the production of triglycerides in HepG2 cells stronger than the curcumin in a concentration dependent manner. The greatest inhibition of TG production shown by concentration of 250 μgmL, while the lowest inhibition shown by the smallest concentration of 15.63 µgmL. Web14 apr. 2024 · Cell lysates were prepared from 2–3 × 10 7 cells using RIP lysis buffer (0.5% NP-40, 20 mM Tris pH 7.4, 150 mM NaCl, and 1.5 mM MgCl 2 in DEPC-H 2 O …
Web9 uur geleden · TOTUM-070 is a patented polyphenol-rich blend of five different plant extracts showing separately a latent effect on lipid metabolism and potential synergistic properties. In this study, we investigated the health benefit of such a formula. Using a preclinical model of high fat diet, TOTUM-070 (3 g/kg of body weight) limited the HFD …
Web5 okt. 2010 · Incubation of HepG2 cells with palmitate markedly increased lipid accumulation (Oil Red O staining), the genes involved in lipogenesis, including fatty acid synthase (FAS) and its upstream regulator sterol regulatory element binding protein 1c (SREBP-1c), and … i\u0027m leaving you for the last time babyWebAs shown in Figure 2A, treatment with increasing doses of isoquercitrin consistently decreased Oil-Red-O staining when compared with that in FFA-only treated samples. Oil-Red-O quantitation by eluate absorbance normalized to total cell number revealed a dose-dependent decrease with isoquercitrin co-treatment ( Figure 2 B). netspend vs walmart money cardWebThe HepG2 cells were treated with control medium and 1.16% ethanol (CTRL), PAL (250 µM) alone or associated with total lipophilic extract (PAL + TLE, 100 µg/mL) or carotenoid extract (PAL + CE, 6 µg/mL) for 24 h before RNA extraction. mRNA levels of genes were quantified using RT-qPCR. i\\u0027m legally blind sound effectWeb25 sep. 2024 · Background Increased consumption of omega-3 (ω-3) fatty acids found in cold-water fish and fish oil has been reported to protect against obesity. A potential mechanism may be through reduction in adipocyte differentiation. Stearidonic acid (SDA), a plant-based ω-3 fatty acid, has been targeted as a potential surrogate for fish-based fatty … netspend want to file disputeWebThe HepG2 cell model of OAinduced intracellular lipid accumulation was developed as previously describedby ChavesTapia et al., [21]. HepG2 cells were cultured with 1mM of OA for 48h, presence and absence of ERAbE (0.1mg/ml) or quercetin (50µM). Oil Red O (ORO) cell staining . Lipid droplets were detected by fluorescent microscopy. netspend walmart cardWeb21 nov. 2024 · Methods: HepG2 cells were treated with palmitate, or palmitate in combination with CCAAT/enhancer binding protein (C/EBP)β siRNA or G0S2 siRNA. The … i\\u0027m lending money and want to hold deedWebMaintenance of HepG2 cells before spheroid generation After thawing from liquid nitrogen, cells were maintained in Nunclon Delta T25 cell culture flasks in Gibco MEM medium supplemented with 10% Gibco FBS and 1% Pen-Strep for 2 passages before seeding for spheroid generation. ATCC protocol was followed for subculturing. Materials required i\\u0027m liberal but to a degree