Jedi-2p
WebJEDI-2P has faster kinetics compared with ASAP2s and ASAP3, related to Figure 2 The voltage of HEK293A cells was modulated under whole cell voltage clamp. Depolarization … WebStar Wars: Jedi Knight: Dark Forces II is a first-person shooter computer game released on October 9, 1997, by LucasArts. This was the first game in the series to include …
Jedi-2p
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Web18 ago 2024 · JEDI-2P is a faster, brighter, more sensitive, and photostable voltage indicator • JEDI-2P was engineered using a two-photon multiparameter screening platform • JEDI-2P enabled two-photon voltage recordings in retinal explants, flies, and mice • JEDI-2P produced deep (cortical layer 5) and long (>40 min) recordings in mice Summary Web24 gen 2024 · Using a low repetition-rate laser, we perform recordings from up to ten neurons simultaneously. Finally, by co-expressing JEDI-2P-kv and the channelrhodopsin ChroME-ST in neurons of hippocampal organotypic slices, we perform single-beam, simultaneous, two-photon voltage imaging and photostimulation.
WebDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a) Depositor … Web6 ott 2024 · Fig. 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The …
WebUsing a novel high-throughput screening platform, we developed JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 minutes using two-photon resonant … Web24 gen 2024 · Using a low repetition-rate laser, we perform recordings from up to ten neurons simultaneously. Finally, by co-expressing JEDI-2P-kv and the channelrhodopsin ChroME-ST in neurons of hippocampal organotypic slices, we perform single-beam, simultaneous, two-photon voltage imaging and photostimulation.
Web7 feb 2024 · JEDI-2P (manuscript submitted) was cloned in a Cre-dependent (floxed) Adeno-Associated Virus (AAV) expression vector with the strong constitutive promoter eF1α (Addgene plasmid #20298). hChR2(H134R)-EYFP in the original vector was replaced with JEDI-2P using standard molecular biology techniques, resulting in the vector pAAV-eF1α …
WebFinally, by co-expressing JEDI-2P-kv and the channelrhodopsin ChroME-ST in neurons of hippocampal organotypic slices, we perform single-beam, simultaneous, two-photon voltage imaging and ... marine supply winter haven floridaWebJEDI-2P: none: Cre dependent: 1: St-Pierre: 5-HT Sensors: 140552: pAAV-hsyn-GRAB_5-HT1.0: Syn: GRAB_5-HT1.0: none: Constitutive: 9: Li: No available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova and Schaffer labs. marine supply store astoriamarine supply store erathWebJEDI-2P, the indicator we report in our Cell paper, is available in various mammalian and insect expression vectors. Search for "JEDI-2P" in our Addgene materials. … marine supply stores seattleWebJEDI-2P was expressed in starburst amacrine cells (SAC, green). GCL, ganglion cell layer and IPL, inner plexiform layer. Visual stimuli were presented to the photoreceptors in the … marine supplys near meWebJEDI-2P 还可以使用共振扫描和 ULoVE 随机访问显微镜以光学方式记录清醒行为小鼠中单个皮层神经元的电压动态超过 30 分钟。 最后，JEDI-2P 的 ULoVE 记录可以稳健地检测深度超过 400 μm 的尖峰，并报告神经元对中的电压相关性。 "点击查看英文标题和摘要" 更新日期：2024-08-18 点击分享 查看原文 点击收藏 公开下载 阅读更多本刊最新论文 本刊介绍/投 … marine supply stores nearbyWebWe demonstrate voltage recordings of high frequency spike trains and sub-threshold depolarizations in intact brain tissue from neurons expressing the soma-targeted … marine support shirts